Oligosaccharides from Marine Algae-III. Purification and Structure of Oligosaccharides from Porphyran degradated by Enzymes from Arthrobacter sp. S-22.
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چکیده
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Purification and Properties of Chitinase from Arthrobacter sp. NHB-10.
A chitinase was purified from the culture filtrate of nigeran-degrading Arthrobacter sp. NHB-10 by precipitation with ammonium sulfate and column chromatographies on DEAE-Sephadex A-50 and Superose 12. The final preparation was homogenous in polyacrylamide gel electrophoresis. The molecular weight of the purified enzyme was 30,000 and its isoelectric point was 6.8. The optimum pH and temperatur...
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ژورنال
عنوان ژورنال: NIPPON SUISAN GAKKAISHI
سال: 1998
ISSN: 1349-998X,0021-5392
DOI: 10.2331/suisan.64.88